Clinical Biochemistry

Investigations	Collection Tube / Sample Type
ACE- Angiotensin Converting Enzyme	Plain Tube / Serum
Acid Phosphatase with Prostatic fraction	Plain Tube / Serum
ADA	Plain Tube / Serum
Ammonia	EDTA or Heparin Plasma (Ice Pack)
Amylase / Lipase	Plain Tube / Serum
Blood Sugar Fasting + PM	Fluoride / Plasma
BUN- Blood Urea Nitrogen	Plain Tube / Serum
Ceruloplasmin	Plain Tube / Serum
Chloride	Plain Tube / Serum
Cholinesterase	Plain Tube / Serum
Copper	Plain Tube / Serum
CPK- MB/ CPK Total	Plain Tube / Serum
G6PD (Quantitative)	EDTA / Whole Blood
Gamma GT	Plain Tube / Serum
Glucose Tolerance Test (GTT)	Fluoride / Plasma
HBA1c	EDTA / Whole Blood
Ionic Calcium	Plain Tube / Serum
Iron / TIBC	Plain Tube / Serum
Iron + TIBC	Plain Tube / Serum
Kidney Function Test	Plain Tube / Serum
Lactate	EDTA / Plasma (Ice Pack)
LDH	Plain Tube / Serum
LDL / VLDL	Plain Tube / Serum
Lipid Profile (Complete)	Plain Tube / Serum
Lipoprotein(a)	Plain Tube / Serum
Liver Function Test	Plain Tube / Serum
Phosphorus, Magnesium	Plain Tube / Serum
Plasma Osmolarity	Plain Tube / Serum
Procalcitonin	Plain Tube / Serum
SGOT, SGPT, Cholesterol, HDL Cholesterol,
Total Bilirubin + Direct / Indirect	Plain Tube / Serum
Total Calcium	Plain Tube / Serum
Total Proteins + Albumin / Globulin	Plan Tube / Serum
Triglycerides	Plain Tube / Serum
Urea, Creatinine, Sodium, Potassium,	Plain Tube / Serum
Uric Acid, Alk Phosphatase

Clinical Pathology

Investigations	Sample Type
Urine Examination
Urine Routine (Alb+ Sug + Micro)	Urine
Urine Ketones	Urine
Urine Osmolarity	Urine
Urine Bile Salts + Bile Pigments	Urine
Urine BJ Proteins	Urine
Urine Pregnancy Test	Urine
Urine Creatinine – Spot	Urine
Urine Creatinine – 24 hrs	Urine – 24 Hours
Urine Protein – Spot	Urine
Urine Proteins – 24 hrs	Urine – 24 Hours
Urine Calcium – Spot	Urine
Urine Calcium – 24 hrs	Urine – 24 Hours
Urine Phosphorus – Spot	Urine
Urine Phosphorus – 24 hrs	Urine – 24 Hours
Urine Sodium – Spot	Urine
Urine Potassium – Spot	Urine
Urine Microalbumin (Colorimetric Pyrogallol Method)	Urine
Urine Microalbumin (Immunoturbidometry Method)	Urine
Urine Protein / Creatinine Ratio	Urine
Urine Copper Level	Urine
Urine VMA	Urine
Urine Myoglobulin	Urine
Urine Chyle	Urine
Urine Cotinine	Urine
Urine Protein Electrophoresis	Urine
Stool Examination
Stool Routine	Stool
Stool Occult Blood	Stool
Stool Routine with Occult Blood	Stool
Stool Reducing Substances	Stool
Stool Hanging Drop	Stool
Others
Fluid Examination (CSF, Pleural, Peritoneal, Pericardial, Synovial)	Sterile Container
Mantoux Test	-
Semen Analysis	Clean Container

Clinical Haematology

Investigations	Collection Tube / Sample Type
Absolute Eosinophil Counts	EDTA / Whole Blood
Absolute Neutrophil Counts	EDTA / Whole Blood
Absolute Lymphocyte Counts	EDTA / Whole Blood
Blood Group and Rh Type	EDTA / Whole Blood
Bone Marrow Aspiration	-
BT, CT (Bleeding Time, Clotting Time )	EDTA / Whole Blood
Complete Haemogram	EDTA / Whole Blood
Direct Coombs Test (Gel Technology)	EDTA / Whole Blood
Haemoglobin, ESR, TLC, DLC	EDTA / Whole Blood
Indirect Coombs Test (Gel Technology)	Plain Tube / Serum
LE Cells	EDTA / Whole Blood
Lupus Anticoagulant	Sodium Citrate / Plasma
Platelet Count	EDTA / Whole Blood
PS for MP	EDTA / Whole Blood
PS for Opinion	EDTA / Whole Blood
Sickling Test, Reticulocyte Count	EDTA / Whole Blood
Coagulation Studies
Anti Thrombin Antibody	Plain Tube / Serum
D Dimer	Sodium Citrate / Plasma
Factor VIII Assay	Sodium Citrate / Plasma
Fibrinogen Assay	Sodium Citrate / Plasma
Protein C	Plain Tube / Serum
Protein S	Plain Tube / Serum
Prothrombin Time/ APTT	Sodium Citrate / Plasma

Microbiology

Sr. No.	INVESTIGATIONS
	STAINS
1	Albert stain
2	Conjunctival Swab
3	Gram Stain
4	Hanging Drop
5	India ink
6	KOH Mount
7	Modified ZN stain
8	PCP stain
9	ZN Stain
10	ZN stain after Decontamination
	CULTURES MANUAL
11	Blood Culture (Manual)
12	Fluids Culture
13	Fungal Culture
14	Pus Culture
15	Sputum Culture
16	Stool Culture
17	TB Culture
18	Urine Culture
	CULTURES AUTOMATED
19	Automated TB Culture (MGIT)
20	Bact Alert + Routine Sensitivity
21	Bact Alert + Vitek Sensitivity
22	TB DST 1st line (Automated –MGIT)
23	TB DST 2nd line (Automated –MGIT)
24	Vitek AST only
	OPERATION THEATRE CARE
25	Air settle plate
26	Swab Culture
	Disinfectants / Water Testing
27	Disinfectants
28	Water

Serology & Infectious Diseases ELISA and Other ELISA

Investigations	Collection Tube / Sample
ASO Titre	Plain Tube / Serum
C Reactive Protein	Plain Tube / Serum
Chikungunya IgM Antibody	Plain Tube / Serum
Dengue IgG+ IgM Antibody	Plain Tube / Serum
Dengue NS1 Antigen	Plain Tube / Serum
Dengue NS1 Antigen + IgG/IgM Antibody	Plain Tube / Serum
Filaria Antigen Card Test	EDTA / Whole Blood
HbsAg Card Test	Plain Tube / Serum
HCV Card Test	Plain Tube / Serum
HIV Card Test	Plain Tube / Serum
hS-CRP	Plain Tube / Serum
IHA for E. histolytica	Plain Tube / Serum
Leptospira IgG + IgM Antibody	Plain Tube / Serum
Malaria Antigen Card Test	EDTA / Whole Blood
RA Test for Rheumatoid Factor (Quantitative)	Plain Tube / Serum
RPR- Rapid Plasma Reagin Test (Qualitative)	Plain Tube / Serum
RPR- Rapid Plasma Reagin Test (Quantitative)	Plain Tube / Serum
Salmonella Antigen Test	Plain Tube / Serum
TPHA- Treponema Pallidum Haemagglutination T	Plain Tube / Serum
VDRL (Qualitative)	Plain Tube / Serum
VDRL (Quantitative)	Plain Tube / Serum
Widal Test (Slide)	Plain Tube / Serum
Widal Test (Tube)	Plain Tube / Serum
Infectious Diseases ELISA
Clostrium difficile	Stool
Scrub Typhus IgM	Plain Tube / Serum
TB Quantiferon Gold Assay	Special Vacutainers
Other ELISA
ACA- Anti-Cardiolipin Antibody, APA-Anti-Phospholipid Antibody	Plain Tube / Serum
AMH- Anti Mullerian Hormone	Plain Tube / Serum
ANA	Plain Tube / Serum
ANA Profile	Plain Tube / Serum
C-ANCA, P-ANCA	Plain Tube / Serum
ds-DNA	Plain Tube / Serum
Ig A- Immunoglobulin A	Plain Tube / Serum
Ig E- Immunoglobulin E	Plain Tube / Serum
Ig G- Immunoglobulin G	Plain Tube / Serum
Ig M- Immunoglobulin M	Plain Tube / Serum
Immunoglobulin	Plain Tube / Serum

IMMUNO ASSAY

Different Markers and Panels done only by Chemiluminescence Method (CMIA) on ARCHITECT, ABBOTT

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	NAME OF THE TEST	Collection Tube / Sample Type
HEPATITIS MARKERS
HEPATITIS A	Anti HAV IgM	Plain Tube / Serum
HEPATITIS B	HBs Ag	Plain Tube / Serum
	Anti HBs	Plain Tube / Serum
	HBe Ag	Plain Tube / Serum
	Anti HBe	Plain Tube / Serum
	Anti HBc IgM	Plain Tube / Serum
	Anti HBc Total	Plain Tube / Serum
HEPATITIS C	Anti HCV Ab	Plain Tube / Serum
HEPATITIS E	HEV IgM Card	Plain Tube / Serum
	HEV IgM ELISA	Plain Tube / Serum
TORCH PANEL
IgM Panel	Toxoplasma IgM	Plain Tube / Serum
	Rubella IgM	Plain Tube / Serum
	CMV IgM	Plain Tube / Serum
	Herpes IgM	Plain Tube / Serum
	TORCH (Total IgM)	Plain Tube / Serum
IgG Panel	Toxoplasma IgG	Plain Tube / Serum
	Rubella IgG	Plain Tube / Serum
	CMV IgG	Plain Tube / Serum
	Herpes IgG	Plain Tube / Serum
	TORCH (Total IgG )	Plain Tube / Serum
HIV
	HIV Duo	Plain Tube / Serum
	HIV ELISA	Plain Tube / Serum
THYROID HORMONES
	T3	Plain Tube / Serum
	T4	Plain Tube / Serum
	TSH	Plain Tube / Serum
	T3 T4 TSH- Thyroid Function Test	Plain Tube / Serum
	FT4, FT3	Plain Tube / Serum
* THYROID ANTIBODIES
	Anti Mitochondrial Antibody	Plain Tube / Serum
	Anti Thyroid Antibody	Plain Tube / Serum
	Anti Thyroglobulin Antibody	Plain Tube / Serum
	Anti Microsomal Antibody (AMA)	Plain Tube / Serum
	Anti Mitochondrial Antibody	Plain Tube / Serum
	Anti TPO Antibody	Plain Tube / Serum
PARATHYROID H
	PTH- Parathyroid Hormone	Plain Tube / Serum
	Calcitonin	Plain Tube / Serum
FERTILITY MARKERS
	β HCG	Plain Tube / Serum
	LH	Plain Tube / Serum
	FSH	Plain Tube / Serum
	Testosterone	Plain Tube / Serum
	Free Testosterone	Plain Tube / Serum
	Prolactin	Plain Tube / Serum
	DHEAS	Plain Tube / Serum
	Estrogen	Plain Tube / Serum
	E2- Estradiol	Plain Tube / Serum
	E3- Estriol	Plain Tube / Serum
	Progesterone	Plain Tube / Serum
	Triple Marker (E2, AFP, B-HCG)	Plain Tube / Serum
	Dual Marker (HCG, PAPP-A)	Plain Tube / Serum
	Quadruple Marker (AFP, B-HCG, E3, Inhibin)	Plain Tube / Serum
OTHER HORMONES
	Insulin	Plain Tube / Serum
	Cortisol	Plain Tube / Serum
	Growth Hormone	Plain Tube / Serum
TUMOR MARKERS
	PSA- Prostatic Specific Antigen	Plain Tube / Serum
	Free PSA	Plain Tube / Serum
	CEA- Carcino Embryonic Antigen	Plain Tube / Serum
	AFP- Alpha Feto Protein	Plain Tube / Serum
	CA 15.3	Plain Tube / Serum
	CA 19.9	Plain Tube / Serum
	CA 125	Plain Tube / Serum
	β 2 Microglobulin	Plain Tube / Serum
TUMOR MARKERS
	Troponin I	Plain Tube / Serum
	Homocysteine	Plain Tube / Serum
	BNP	Plain Tube / Serum
DRUG MONITORING(TDM)
	Phenytoin	Plain Tube / Serum
	Digoxin	Plain Tube / Serum
	*Lithium	Plain Tube / Serum
MISCELLANEOUS CMIA
	Ferritin	Plain Tube / Serum
	Folic Acid	Plain Tube / Serum
	Vit B12	Plain Tube / Serum
	Vit D3	Plain Tube / Serum
	Anti CCP	Plain Tube / Serum
ELECTROPHORESIS
	Haemoglobin Electrophoresis	EDTA / Whole Blood
	HbA2 Quantification	EDTA / Whole Blood
	Serum Electrophoresis	Plain Tube / Serum
	Urine for M Band	Urine
	ABG	Heparin / Whole Blood
	VBG	Heparin / Whole Blood
	Bicarbonate	Heparin / Whole Blood
	C3	Plain Tube / Serum
	C4	Plain Tube / Serum
	C Peptide	Plain Tube / Serum
	Acetyl Choline Receptor Antibody	Plain Tube / Serum

Special Tests

	Name of Investigation	Collection Details
P C R (Polymerase Chain Reaction)	EDTA Blood, Body Fluid / Sputum in plain bulb Tissue / FNAC material in Normal Saline
H1N1 Influenza Real Time PCR	Throat/ Nasal swab (in VTM)
Hepatitis B (HBV) DNA PCR (Qualitative)	EDTA + Plain Tube
Hepatitis B (HBV) DNA PCR (Quantitative)	EDTA + Plain Tube
Hepatitis C (HCV) PCR (Qualitative)	EDTA + Plain Tube
Hepatitis C (HCV) RNA PCR (Quantitative)	EDTA + Plain Tube
Herpes Simplex Virus I & II (HSV I/II) PCR	Plain Tube
Human immunodeficiency Virus (HIV) PCR (Qualitative)	EDTA / Whole Blood
Human immunodeficiency Virus (HIV) (Quantitative)	EDTA / Whole Blood
Human Papilloma Virus (HPV) DNA PCR	Cervical Scrape in saline
Tuberculosis Nested PCR	Plain Tube
Flow Cytometer
CD4 Counts	EDTA / Whole Blood
HLA-B27	EDTA / Whole Blood
Kidney Transplant Work Up
HLA Typing (A, B DR: SSP Method)	EDTA / Whole Blood
Lymphocyte Cross Match	Donor EDTA / Whole Blood Recipient Plain Tube / Serum
Donor Specific Antibody (DSA)	Recipient EDTA + Plain Tube (2)
HLA Ab Screen Class I and II	Recipient EDTA + Plain Tube (2)
IHC (Immunohistochemistry)
ER + PgR ER + PgR + Her 2 neu	Paraffin blocks / formalin fixed tissues.
Histopathology
Small Specimen	10% Formalin
Medium Specimen	10% Formalin
Large Specimen	10% Formalin
Cytology
Pap Smear
FNAC	Alcohol Fixed